PI3 Kinase p110 beta Antibody

ProSci
Product Code: PSI-13-351
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-351-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PI3 Kinase p110 beta antibody (13-351) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
2 / 4
Immunohistochemistry of paraffin-embedded mouse lung using PI3 Kinase p110 beta antibody (13-351) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human lung using PI3 Kinase p110 beta antibody (13-351) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded human colon carcinoma using PI3 Kinase p110 beta antibody (13-351) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using PI3 Kinase p110 beta antibody (13-351) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded mouse lung using PI3 Kinase p110 beta antibody (13-351) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using PI3 Kinase p110 beta antibody (13-351) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using PI3 Kinase p110 beta antibody (13-351) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
PIK3CB, PI3K, PIK3C1, P110BETA, PI3KBETA
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene encodes an isoform of the catalytic subunit of phosphoinositide 3-kinase (PI3K). These kinases are important in signaling pathways involving receptors on the outer membrane of eukaryotic cells and are named for their catalytic subunit. The encoded protein is the catalytic subunit for PI3Kbeta (PI3KB). PI3KB has been shown to be part of the activation pathway in neutrophils which have bound immune complexes at sites of injury or infection. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 771-1070 of human PI3 Kinase p110 beta (NP_006210.1).
NCBI Gene ID #:
5291
NCBI Official Name:
phosphatidylinositol-4,5-bisphosphate 3-kinase, catalytic subunit beta
NCBI Official Symbol:
PIK3CB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 122kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Immunology, Innate Immunity, Signal Transduction
Swissprot #:
P42338
User NOte:
Optimal dilutions for each application to be determined by the researcher.