NEK8 Antibody

ProSci
Product Code: PSI-13-353
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-353-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using NEK8 antibody (13-353) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunofluorescence analysis of HeLa cells using NEK8 antibody (13-353). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using NEK8 antibody (13-353) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of HeLa cells using NEK8 antibody (13-353). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
Serine/threonine-protein kinase Nek8, Never in mitosis A-related kinase 8, NimA-related protein kinase 8, Nima-related protein kinase 12a, NEK8, JCK, NEK12A
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a member of the serine/threionine protein kinase family related to NIMA (never in mitosis, gene A) of Aspergillus nidulans. The encoded protein may play a role in cell cycle progression from G2 to M phase. Mutations in the related mouse gene are associated with a disease phenotype that closely parallels the juvenile autosomal recessive form of polycystic kidney disease in humans.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human NEK8 (NP_835464.1).
NCBI Gene ID #:
284086
NCBI Official Name:
Serine/threonine-protein kinase Nek8
NCBI Official Symbol:
NEK8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 80kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
Q86SG6
User NOte:
Optimal dilutions for each application to be determined by the researcher.