Hexokinase II Antibody

ProSci
Product Code: PSI-13-360
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-360-50uL50uL£440.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using Hexokinase II antibody (13-360) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded human breast cancer using Hexokinase II antibody (13-360) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using Hexokinase II antibody (13-360) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using Hexokinase II antibody (13-360) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded rat heart using Hexokinase II antibody (13-360) at dilution of 1:200 (40x lens).
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Immunofluorescence analysis of U2OS cells using Hexokinase II antibody (13-360). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using Hexokinase II antibody (13-360) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human breast cancer using Hexokinase II antibody (13-360) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using Hexokinase II antibody (13-360) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using Hexokinase II antibody (13-360) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded rat heart using Hexokinase II antibody (13-360) at dilution of 1:200 (40x lens).
Immunofluorescence analysis of U2OS cells using Hexokinase II antibody (13-360). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
HK2, DKFZp686M1669, HKII, HXK2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Hexokinases phosphorylate glucose to produce glucose-6-phosphate, the first step in most glucose metabolism pathways. This gene encodes hexokinase 2, the predominant form found in skeletal muscle. It localizes to the outer membrane of mitochondria. Expression of this gene is insulin-responsive, and studies in rat suggest that it is involved in the increased rate of glycolysis seen in rapidly growing cancer cells.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human Hexokinase II (NP_000180.2).
NCBI Gene ID #:
3099
NCBI Official Name:
hexokinase 2
NCBI Official Symbol:
HK2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 102kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P52789
User NOte:
Optimal dilutions for each application to be determined by the researcher.