ID2 Antibody

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ProSci
Product Code: PSI-13-362
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-362-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse lung, using ID2 antibody (13-362) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat ovary using ID2 antibody (13-362) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human oophoroma using ID2 antibody (13-362) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using ID2 antibody (13-362) at dilution of 1:100 (40x lens).
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Western blot analysis of extracts of mouse lung, using ID2 antibody (13-362) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat ovary using ID2 antibody (13-362) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human oophoroma using ID2 antibody (13-362) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using ID2 antibody (13-362) at dilution of 1:100 (40x lens).

Documents

Further Information

Additional Names:
ID2, GIG8, ID2A, ID2H, MGC26389, bHLHb26
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
The protein encoded by this gene belongs to the inhibitor of DNA binding family, members of which are transcriptional regulators that contain a helix-loop-helix (HLH) domain but not a basic domain. Members of the inhibitor of DNA binding family inhibit the functions of basic helix-loop-helix transcription factors in a dominant-negative manner by suppressing their heterodimerization partners through the HLH domains. This protein may play a role in negatively regulating cell differentiation. A pseudogene of this gene is located on chromosome 3.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 75-134 of human ID2 (NP_002157.2).
NCBI Gene ID #:
3398
NCBI Official Name:
inhibitor of DNA binding 2, dominant negative helix-loop-helix protein
NCBI Official Symbol:
ID2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 15kDa
Purification:
Affinity purification
Research Area:
Stem Cell, Transcription
Swissprot #:
Q02363
User NOte:
Optimal dilutions for each application to be determined by the researcher.