ARRB1 Antibody

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ProSci
Product Code: PSI-13-363
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-363-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ARRB1 antibody (13-363) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunofluorescence analysis of HeLa cells using ARRB1 antibody (13-363). Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 200ug extracts of K-562 cells, using 3 ug ARRB1 antibody (13-363). Western blot was performed from the immunoprecipitate using ARRB1 antibody (13-363) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using ARRB1 antibody (13-363) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of HeLa cells using ARRB1 antibody (13-363). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of K-562 cells, using 3 ug ARRB1 antibody (13-363). Western blot was performed from the immunoprecipitate using ARRB1 antibody (13-363) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
Beta-arrestin-1, Arrestin beta-1, ARRB1, ARR1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:20 - 1:100

IP: 1:50 - 1:100
Background:
Members of arrestin/beta-arrestin protein family are thought to participate in agonist-mediated desensitization of G-protein-coupled receptors and cause specific dampening of cellular responses to stimuli such as hormones, neurotransmitters, or sensory signals. Arrestin beta 1 is a cytosolic protein and acts as a cofactor in the beta-adrenergic receptor kinase (BARK) mediated desensitization of beta-adrenergic receptors. Besides the central nervous system, it is expressed at high levels in peripheral blood leukocytes, and thus the BARK/beta-arrestin system is believed to play a major role in regulating receptor-mediated immune functions. Alternatively spliced transcripts encoding different isoforms of arrestin beta 1 have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 169-418 of human ARRB1 (NP_004032.2).
NCBI Gene ID #:
408
NCBI Official Name:
Beta-arrestin-1
NCBI Official Symbol:
ARRB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 56kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
P49407
User NOte:
Optimal dilutions for each application to be determined by the researcher.