HPCAL1 Antibody

ProSci
Product Code: PSI-13-378
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-378-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HPCAL1 antibody (13-378) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
2 / 2
Immunoprecipitation analysis of extracts of HepG2 cells using HPCAL1 antibody (13-378). Western blot was performed from the immunoprecipitate using HPCAL1 antibody (13-378) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using HPCAL1 antibody (13-378) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunoprecipitation analysis of extracts of HepG2 cells using HPCAL1 antibody (13-378). Western blot was performed from the immunoprecipitate using HPCAL1 antibody (13-378) at a dilition of 1:1000.

Further Information

Additional Names:
BDR1, HLP2, VILIP-3, hippocalcin-like protein 1, calcium-binding protein BDR-1, visinin-like protein 3
Application Note:
WB: 1:500 - 1:2000

IP: 1:20 - 1:100
Background:
The protein encoded by this gene is a member of neuron-specific calcium-binding proteins family found in the retina and brain. It is highly similar to human hippocalcin protein and nearly identical to the rat and mouse hippocalcin like-1 proteins. It may be involved in the calcium-dependent regulation of rhodopsin phosphorylation and may be of relevance for neuronal signalling in the central nervous system. Several alternatively spliced transcript variants encoding the same protein have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human HPCAL1 (NP_002140.2).
NCBI Gene ID #:
3241
NCBI Official Name:
hippocalcin like 1
NCBI Official Symbol:
HPCAL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 17kDa
Purification:
Affinity purification
Research Area:
Neuroscience
Swissprot #:
P37235
User NOte:
Optimal dilutions for each application to be determined by the researcher.