RPL8 Antibody

ProSci
Product Code: PSI-13-394
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-394-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using RPL8 antibody (13-394) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 3
Immunofluorescence analysis of HeLa cells using RPL8 antibody (13-394) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of HeLa cells using RPL8 antibody (13-394) at dilution of 1:200. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using RPL8 antibody (13-394) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunofluorescence analysis of HeLa cells using RPL8 antibody (13-394) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using RPL8 antibody (13-394) at dilution of 1:200. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
RPL8, L8
Application Note:
WB: 1:1000 - 1:4000

IF: 1:100 - 1:200
Background:
Ribosomes, the organelles that catalyze protein synthesis, consist of a small 40S subunit and a large 60S subunit. Together these subunits are composed of 4 RNA species and approximately 80 structurally distinct proteins. This gene encodes a ribosomal protein that is a component of the 60S subunit. The protein belongs to the L2P family of ribosomal proteins. It is located in the cytoplasm. In rat, the protein associates with the 5.8S rRNA, very likely participates in the binding of aminoacyl-tRNA, and is a constituent of the elongation factor 2-binding site at the ribosomal subunit interface. Alternatively spliced transcript variants encoding the same protein exist. As is typical for genes encoding ribosomal proteins, there are multiple processed pseudogenes of this gene dispersed through the genome.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-257 of human RPL8 (NP_000964.1).
NCBI Gene ID #:
6132
NCBI Official Name:
ribosomal protein L8
NCBI Official Symbol:
RPL8
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 28kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
P62917
User NOte:
Optimal dilutions for each application to be determined by the researcher.