CYP4F2 Antibody

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ProSci
Product Code: PSI-13-445
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-445-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CYP4F2 antibody (13-445) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Western blot analysis of extracts of various cell lines, using CYP4F2 antibody (13-445) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.

Documents

Further Information

Additional Names:
CPF2, phylloquinone omega-hydroxylase CYP4F2, 20-HETE synthase, 20-hydroxyeicosatetraenoic acid synthase, CYPIVF2, arachidonic acid omega-hydroxylase, cytochrome P450 4F2, cytochrome P45family 4, subfamily F, polypeptide 2, cytochrome P45subfamily IVF, polypeptide 2, cytochrome P450-LTB-omega, leukotriene-B(4) 20-monooxygenase 1, leukotriene-B(4) omega-hydroxylase 1
Application Note:
WB: 1:500 - 1:2000
Background:
This gene encodes a member of the cytochrome P450 superfamily of enzymes. The cytochrome P450 proteins are monooxygenases which catalyze many reactions involved in drug metabolism and synthesis of cholesterol, steroids and other lipids. This protein localizes to the endoplasmic reticulum. The enzyme starts the process of inactivating and degrading leukotriene B4, a potent mediator of inflammation. This gene is part of a cluster of cytochrome P450 genes on chromosome 19. Another member of this family, CYP4F11, is approximately 16 kb away.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 40-120 of human CYP4F2 (NP_001073.3).
NCBI Gene ID #:
8529
NCBI Official Name:
cytochrome P450 family 4 subfamily F member 2
NCBI Official Symbol:
CYP4F2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 60kDa
Purification:
Affinity purification
Research Area:
Cancer, Immunology, Innate Immunity, Signal Transduction
Swissprot #:
P78329
User NOte:
Optimal dilutions for each application to be determined by the researcher.