AP1M1 Antibody

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ProSci
Product Code: PSI-13-446
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-446-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using AP1M1 antibody (13-446) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Western blot analysis of extracts of various cell lines, using AP1M1 antibody (13-446) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.

Documents

Further Information

Additional Names:
AP-1 complex subunit mu-1, AP-mu chain family member mu1A, Adaptor protein complex AP-1 subunit mu-1, Adaptor-related protein complex 1 subunit mu-1, Clathrin assembly protein complex 1 mu-1 medium chain 1, Clathrin coat assembly protein AP47, Clathrin coat-associated protein AP47, Golgi adaptor HA1/AP1 adaptin mu-1 subunit, Mu-adaptin 1, Mu1A-adaptin, AP1M1, CLTNM
Application Note:
WB: 1:1000 - 1:4000
Background:
The protein encoded by this gene is the medium chain of the trans-Golgi network clathrin-associated protein complex AP-1. The other components of this complex are beta-prime-adaptin, gamma-adaptin, and the small chain AP1S1. This complex is located at the Golgi vesicle and links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. Alternatively spliced transcript variants encoding distinct protein isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human AP1M1 (NP_115882.1).
NCBI Gene ID #:
8907
NCBI Official Name:
AP-1 complex subunit mu-1
NCBI Official Symbol:
AP1M1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 48kDa
Purification:
Affinity purification
Research Area:
Immunology, Signal Transduction
Swissprot #:
Q9BXS5
User NOte:
Optimal dilutions for each application to be determined by the researcher.