RPS6KA4 Antibody

ProSci
Product Code: PSI-13-447
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-447-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of various cell lines, using RPS6KA4 antibody (13-447) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
2 / 2
Immunohistochemistry of paraffin-embedded mouse lung using RPS6KA4 antibody (13-447) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of various cell lines, using RPS6KA4 antibody (13-447) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded mouse lung using RPS6KA4 antibody (13-447) at dilution of 1:200 (40x lens).

Further Information

Additional Names:
Ribosomal protein S6 kinase alpha-4, S6K-alpha-4, 90 kDa ribosomal protein S6 kinase 4, Nuclear mitogen- and stress-activated protein kinase 2, Ribosomal protein kinase B, RSKB, RPS6KA4, MSK2
Application Note:
WB: 1:200 - 1:1000

IHC: 1:20 - 1:100

IF: 1:20 - 1:100
Background:
This gene encodes a member of the RSK (ribosomal S6 kinase) family of serine/threonine kinases. This kinase contains 2 non-identical kinase catalytic domains and phosphorylates various substrates, including CREB1 and ATF1. The encoded protein can also phosphorylate histone H3 to regulate certain inflammatory genes. Several transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-220 of human RPS6KA4 (NP_003933.1).
NCBI Gene ID #:
8986
NCBI Official Name:
Ribosomal protein S6 kinase alpha-4
NCBI Official Symbol:
RPS6KA4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 115kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Immunology, Signal Transduction
Swissprot #:
O75676
User NOte:
Optimal dilutions for each application to be determined by the researcher.