CLUH Antibody

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ProSci
Product Code: PSI-13-454
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-454-50uL50uL£433.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CLUH antibody (13-454) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
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Immunohistochemistry of paraffin-embedded human placenta using CLUH antibody (13-454) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human stomach using CLUH antibody (13-454) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using CLUH antibody (13-454) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of NIH-3T3 cells using CLUH Polyclonal Antibody (13-454) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U-2 OS cells using CLUH Polyclonal Antibody (13-454) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using CLUH antibody (13-454) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded human placenta using CLUH antibody (13-454) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using CLUH antibody (13-454) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using CLUH antibody (13-454) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH-3T3 cells using CLUH Polyclonal Antibody (13-454) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using CLUH Polyclonal Antibody (13-454) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
CLU1, clustered mitochondria protein homolog, clustered mitochondria (cluA/CLU1) homolog
Application Note:
WB: 1:1000 - 1:4000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1120-1309 of human CLUH (NP_056044.3).
NCBI Gene ID #:
23277
NCBI Official Name:
clustered mitochondria homolog
NCBI Official Symbol:
CLUH
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 180kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
O75153
User NOte:
Optimal dilutions for each application to be determined by the researcher.