PPP1R14A Antibody

ProSci
Product Code: PSI-13-473
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-473-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using PPP1R14A antibody (13-473) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.

Western blot analysis of extracts of various cell lines, using PPP1R14A antibody (13-473) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.

Further Information

Additional Names:
CPI-17, CPI17, PPP1INL, protein phosphatase 1 regulatory subunit 14A, 17 kDa PKC-potentiated inhibitory protein of PP1, 17-KDa protein, PKC-potentiated inhibitory protein of PP1, protein kinase C-potentiated inhibitor protein of 17 kDa
Application Note:
WB: 1:500 - 1:2000
Background:
The protein encoded by this gene belongs to the protein phosphatase 1 (PP1) inhibitor family. This protein is an inhibitor of smooth muscle myosin phosphatase, and has higher inhibitory activity when phosphorylated. Inhibition of myosin phosphatase leads to increased myosin phosphorylation and enhanced smooth muscle contraction. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-147 of human PPP1R14A (NP_150281.1).
NCBI Gene ID #:
94274
NCBI Official Name:
protein phosphatase 1 regulatory inhibitor subunit 14A
NCBI Official Symbol:
PPP1R14A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 17kDa
Purification:
Affinity purification
Research Area:
Signal Transduction
Swissprot #:
Q96A00
User NOte:
Optimal dilutions for each application to be determined by the researcher.