CLDN5 Antibody

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ProSci
Product Code: PSI-13-500
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-500-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of mouse lung, using CLDN5 antibody (13-500) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
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Immunofluorescence analysis of NIH/3T3 cells using CLDN5 antibody (13-500) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U2OS cells using CLDN5 antibody (13-500) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of mouse lung, using CLDN5 antibody (13-500) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 60s.
Immunofluorescence analysis of NIH/3T3 cells using CLDN5 antibody (13-500) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using CLDN5 antibody (13-500) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
CLDN5, BEC1, AWAL, Claudin-5, CPETRL1, TMDVCF, TMVCF, Claudin 5
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a member of the claudin family. Claudins are integral membrane proteins and components of tight junction strands. Tight junction strands serve as a physical barrier to prevent solutes and water from passing freely through the paracellular space between epithelial or endothelial cell sheets. Mutations in this gene have been found in patients with velocardiofacial syndrome. Alternatively spliced transcript variants encoding the same protein have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 230-303 of human CLDN5 (NP_001124333.1).
NCBI Gene ID #:
7122
NCBI Official Name:
claudin 5
NCBI Official Symbol:
CLDN5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 27kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
O00501
User NOte:
Optimal dilutions for each application to be determined by the researcher.