EPHB3 Antibody

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ProSci
Product Code: PSI-13-506
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-506-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of HeLa cells, using EPHB3 antibody (13-506) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Western blot analysis of extracts of HeLa cells, using EPHB3 antibody (13-506) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.

Documents

Further Information

Additional Names:
EPHB3, Cek10, ETK2, HEK2 protein kinase, Embryonic kinase 2, EPH receptor B3, EPH-like kinase 2, Ephrin type-B receptor 3, MDK5, Sek4, TYRO6, EK2, EPH-like tyrosine kinase 2, EPH-like tyrosine kinase-2, HEK2, Human embryo kinase 2, Tyrosine-protein kinase TYRO6
Application Note:
WB: 1:500 - 1:2000
Background:
Ephrin receptors and their ligands, the ephrins, mediate numerous developmental processes, particularly in the nervous system. Based on their structures and sequence relationships, ephrins are divided into the ephrin-A (EFNA) class, which are anchored to the membrane by a glycosylphosphatidylinositol linkage, and the ephrin-B (EFNB) class, which are transmembrane proteins. The Eph family of receptors are divided into two groups based on the similarity of their extracellular domain sequences and their affinities for binding ephrin-A and ephrin-B ligands. Ephrin receptors make up the largest subgroup of the receptor tyrosine kinase (RTK) family. This gene encodes a receptor for ephrin-B family members.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 270-560 of human EPHB3 (NP_004434.2).
NCBI Gene ID #:
2049
NCBI Official Name:
EPH receptor B3
NCBI Official Symbol:
EPHB3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 110kDa
Purification:
Affinity purification
Research Area:
Neuroscience, Signal Transduction
Swissprot #:
P54753
User NOte:
Optimal dilutions for each application to be determined by the researcher.