KRT18 Antibody

ProSci
Product Code: PSI-13-511
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-511-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using KRT18 antibody (13-511) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded human liver cancer using KRT18 antibody (13-511) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human small intestine using KRT18 antibody (13-511) at dilution of 1:100 (40x lens).
4 / 4
Immunofluorescence analysis of U2OS cells using KRT18 antibody (13-511) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using KRT18 antibody (13-511) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human liver cancer using KRT18 antibody (13-511) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human small intestine using KRT18 antibody (13-511) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using KRT18 antibody (13-511) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
KRT18, K18, CYK18
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
KRT18 encodes the type I intermediate filament chain keratin 18. Keratin 18, together with its filament partner keratin 8, are perhaps the most commonly found members of the intermediate filament gene family. They are expressed in single layer epithelial tissues of the body. Mutations in this gene have been linked to cryptogenic cirrhosis. Two transcript variants encoding the same protein have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 127-430 of human KRT18 (NP_000215.1).
NCBI Gene ID #:
3875
NCBI Official Name:
keratin 18
NCBI Official Symbol:
KRT18
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 48kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Signal Transduction
Swissprot #:
P05783
User NOte:
Optimal dilutions for each application to be determined by the researcher.