YWHAB Antibody

ProSci
Product Code: PSI-13-518
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-518-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using YWHAB antibody (13-518) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded Human tonsil using YWHAB antibody (13-518) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded Human appendix using YWHAB antibody (13-518) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of various cell lines, using YWHAB antibody (13-518) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded Human tonsil using YWHAB antibody (13-518) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded Human appendix using YWHAB antibody (13-518) at dilution of 1:200 (40x lens).

Further Information

Additional Names:
YWHAB, 14-3-3 alpha, 14-3-3 Beta, 14-3-3 protein beta/alpha, GW128, Protein 1054, HS1, KCIP-1, YWHAA
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100
Background:
This gene encodes a protein belonging to the 14-3-3 family of proteins, members of which mediate signal transduction by binding to phosphoserine-containing proteins. This highly conserved protein family is found in both plants and mammals. The encoded protein has been shown to interact with RAF1 and CDC25 phosphatases, suggesting that it may play a role in linking mitogenic signaling and the cell cycle machinery. Two transcript variants, which encode the same protein, have been identified for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-246 of human YWHAB (NP_647539.1).
NCBI Gene ID #:
7529
NCBI Official Name:
tyrosine 3-monooxygenase/tryptophan 5-monooxygenase activation protein, beta
NCBI Official Symbol:
YWHAB
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 28kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Immunology, Neuroscience, Signal Transduction, Stem Cell
Swissprot #:
P31946
User NOte:
Optimal dilutions for each application to be determined by the researcher.