ASPN Antibody

ProSci
Product Code: PSI-13-571
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-571-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 3
Western blot analysis of extracts of various cell lines, using ASPN antibody (13-571) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 3
Immunofluorescence analysis of rat brain using ASPN antibody (13-571) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
3 / 3
Immunofluorescence analysis of rat heart using ASPN antibody (13-571) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using ASPN antibody (13-571) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunofluorescence analysis of rat brain using ASPN antibody (13-571) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat heart using ASPN antibody (13-571) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
ASPN, FLJ20129, PLAP1, SLRR1C, OS3, PLAP-1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
This gene encodes a cartilage extracellular protein that is member of the small leucine-rich proteoglycan family. The encoded protein may regulate chondrogenesis by inhibiting transforming growth factor-beta 1-induced gene expression in cartilage. This protein also binds collagen and calcium and may induce collagen mineralization. Polymorphisms in the aspartic acid repeat region of this gene are associated with a susceptibility to osteoarthritis, and also with intervertebral disc disease. Alternative splicing of this gene results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 100-379 of human ASPN (NP_060150.4).
NCBI Gene ID #:
54829
NCBI Official Name:
asporin
NCBI Official Symbol:
ASPN
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 43kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
Q9BXN1
User NOte:
Optimal dilutions for each application to be determined by the researcher.