ENO1 Antibody, KO Validated

ProSci
Product Code: PSI-13-583
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-583-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 5
Western blot analysis of extracts of various cell lines, using ENO1 antibody (13-583) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 5
Western blot analysis of extracts from normal (control) and ENO1 knockout (KO) HeLa cells, using ENO1 antibody (13-583) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
3 / 5
Immunofluorescence analysis of NIH-3T3 cells using ENO1 antibody (13-583) at dilution of 1:100. Blue: DAPI for nuclear staining.
4 / 5
Immunofluorescence analysis of U-2 OS cells using ENO1 antibody (13-583) at dilution of 1:100. Blue: DAPI for nuclear staining.
5 / 5
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug ENO1 antibody (13-583). Western blot was performed from the immunoprecipitate using ENO1 antibody (13-583) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using ENO1 antibody (13-583) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and ENO1 knockout (KO) HeLa cells, using ENO1 antibody (13-583) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunofluorescence analysis of NIH-3T3 cells using ENO1 antibody (13-583) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U-2 OS cells using ENO1 antibody (13-583) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug ENO1 antibody (13-583). Western blot was performed from the immunoprecipitate using ENO1 antibody (13-583) at a dilition of 1:1000.

Further Information

Additional Names:
ENO1, NNE, PPH, MPB1, ENO1L1
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100

IP: 1:50 - 1:200
Background:
This gene encodes alpha-enolase, one of three enolase isoenzymes found in mammals. Each isoenzyme is a homodimer composed of 2 alpha, 2 gamma, or 2 beta subunits, and functions as a glycolytic enzyme. Alpha-enolase in addition, functions as a structural lens protein (tau-crystallin) in the monomeric form. Alternative splicing of this gene results in a shorter isoform that has been shown to bind to the c-myc promoter and function as a tumor suppressor. Several pseudogenes have been identified, including one on the long arm of chromosome 1. Alpha-enolase has also been identified as an autoantigen in Hashimoto encephalopathy.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-434 of human ENO1 (NP_001419.1).
NCBI Gene ID #:
2023
NCBI Official Name:
enolase 1, (alpha)
NCBI Official Symbol:
ENO1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 47kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction, Transcription
Swissprot #:
P06733
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.