MMP16 Antibody

ProSci
Product Code: PSI-13-634
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-634-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MMP16 antibody (13-634) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunohistochemistry of paraffin-embedded human lung cancer using MMP16 antibody (13-634) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon carcinoma using MMP16 antibody (13-634) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast using MMP16 antibody (13-634) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using MMP16 antibody (13-634) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of NIH/3T3 cells using MMP16 antibody (13-634) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using MMP16 antibody (13-634) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunohistochemistry of paraffin-embedded human lung cancer using MMP16 antibody (13-634) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon carcinoma using MMP16 antibody (13-634) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using MMP16 antibody (13-634) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using MMP16 antibody (13-634) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using MMP16 antibody (13-634) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
MMP16, C8orf57, MMP-X2, MT-MMP3, MT3MMP, MT-MMP 3, MT-MMP2, MT2-MMP, Matrix metalloproteinase-16, Membrane type 3-MMP, MMP-16, MMPX2, MT3-MMP, MTMMP3
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The encoded protein activates MMP2 by cleavage. This gene was once referred to as MT-MMP2, but was renamed as MT-MMP3 or MMP16.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 290-565 of human MMP16 (NP_005932.2).
NCBI Gene ID #:
4325
NCBI Official Name:
matrix metallopeptidase 16 (membrane-inserted)
NCBI Official Symbol:
MMP16
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 60kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle
Swissprot #:
P51512
User NOte:
Optimal dilutions for each application to be determined by the researcher.