SLC4A5 Antibody

ProSci
Product Code: PSI-13-648
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-648-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using SLC4A5 antibody (13-648) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
2 / 4
Immunohistochemistry of paraffin-embedded rat liver using SLC4A5 antibody (13-648) at dilution of 1:100 (40x lens).
3 / 4
Immunohistochemistry of paraffin-embedded rat kidney using SLC4A5 antibody (13-648) at dilution of 1:100 (40x lens).
4 / 4
Immunohistochemistry of paraffin-embedded human liver using SLC4A5 antibody (13-648) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using SLC4A5 antibody (13-648) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Immunohistochemistry of paraffin-embedded rat liver using SLC4A5 antibody (13-648) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat kidney using SLC4A5 antibody (13-648) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver using SLC4A5 antibody (13-648) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
NBC4, NBCe2, electrogenic sodium bicarbonate cotransporter 4, solute carrier family 4 (sodium bicarbonate cotransporter), member 5
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene encodes a member of the sodium bicarbonate cotransporter (NBC) family, part of the bicarbonate transporter superfamily. Sodium bicarbonate cotransporters are involved in intracellular pH regulation and electroneural or electrogenic sodium bicarbonate transport. This protein is thought to be an integral membrane protein. Multiple transcript variants encoding different isoforms have been found for this gene, but the biological validity of some variants has not been determined.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1042-1121 of human SLC4A5 (NP_597812.1).
NCBI Gene ID #:
57835
NCBI Official Name:
solute carrier family 4 member 5
NCBI Official Symbol:
SLC4A5
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 110kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction
Swissprot #:
Q9BY07
User NOte:
Optimal dilutions for each application to be determined by the researcher.