U2AF1 Antibody

ProSci
Product Code: PSI-13-665
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-665-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using U2AF1 antibody (13-665) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat Intestine using U2AF1 antibody (13-665) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded human oophoroma using U2AF1 antibody (13-665) at dilution of 1:200 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using U2AF1 antibody (13-665) at dilution of 1:200 (40x lens).

Western blot analysis of extracts of various cell lines, using U2AF1 antibody (13-665) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat Intestine using U2AF1 antibody (13-665) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded human oophoroma using U2AF1 antibody (13-665) at dilution of 1:200 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using U2AF1 antibody (13-665) at dilution of 1:200 (40x lens).

Further Information

Additional Names:
Splicing factor U2AF 35 kDa subunit, U2 auxiliary factor 35 kDa subunit, U2 small nuclear RNA auxiliary factor 1, U2 snRNP auxiliary factor small subunit, U2AF1, U2AF35, U2AFBP
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:100
Background:
This gene belongs to the splicing factor SR family of genes. U2 auxiliary factor, comprising a large and a small subunit, is a non-snRNP protein required for the binding of U2 snRNP to the pre-mRNA branch site. This gene encodes the small subunit which plays a critical role in both constitutive and enhancer-dependent RNA splicing by directly mediating interactions between the large subunit and proteins bound to the enhancers. Alternatively spliced transcript variants encoding different isoforms have been identified.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-240 of human U2AF1 (NP_006749.1).
NCBI Gene ID #:
7307
NCBI Official Name:
Splicing factor U2AF 35 kDa subunit
NCBI Official Symbol:
U2AF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 38kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle
Swissprot #:
Q01081
User NOte:
Optimal dilutions for each application to be determined by the researcher.