MTAP Antibody

ProSci
Product Code: PSI-13-684
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-684-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MTAP antibody (13-684) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded rat brain using MTAP antibody (13-684) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse liver using MTAP antibody (13-684) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of A-549 cells using MTAP antibody (13-684). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using MTAP antibody (13-684) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat brain using MTAP antibody (13-684) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using MTAP antibody (13-684) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A-549 cells using MTAP antibody (13-684). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
BDMF, DMSFH, DMSMFH, HEL-249, LGMBF, MSAP, c86fus, S-methyl-5'-thioadenosine phosphorylase, 5'-methylthioadenosine phosphorylase, MTA phosphorylase, MTAPase, MeSAdo phosphorylase, epididymis luminal protein 249, epididymis secretory sperm binding protein
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes an enzyme that plays a major role in polyamine metabolism and is important for the salvage of both adenine and methionine. The encoded enzyme is deficient in many cancers because this gene and the tumor suppressor p16 gene are co-deleted. Multiple alternatively spliced transcript variants have been described for this gene, but their full-length natures remain unknown.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-283 of human MTAP (NP_002442.2).
NCBI Gene ID #:
4507
NCBI Official Name:
methylthioadenosine phosphorylase
NCBI Official Symbol:
MTAP
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 31kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
Q13126
User NOte:
Optimal dilutions for each application to be determined by the researcher.