SMAD7 Antibody

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ProSci
Product Code: PSI-13-698
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-698-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of SW480 cells, using SMAD7 antibody (13-698) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
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Immunofluorescence analysis of U2OS cells using SMAD7 antibody (13-698) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of SW480 cells, using SMAD7 antibody (13-698) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunofluorescence analysis of U2OS cells using SMAD7 antibody (13-698) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
SMAD7, CRCS3, MADH7, MADH8
Application Note:
WB: 1:500 - 1:1000

IF: 1:50 - 1:100
Background:
The protein encoded by this gene is a nuclear protein that binds the E3 ubiquitin ligase SMURF2. Upon binding, this complex translocates to the cytoplasm, where it interacts with TGF-beta receptor type-1 (TGFBR1), leading to the degradation of both the encoded protein and TGFBR1. Expression of this gene is induced by TGFBR1. Variations in this gene are a cause of susceptibility to colorectal cancer type 3 (CRCS3). Several transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 220-390 of human SMAD7 (NP_005895.1).
NCBI Gene ID #:
4092
NCBI Official Name:
SMAD family member 7
NCBI Official Symbol:
SMAD7
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 46kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
O15105
User NOte:
Optimal dilutions for each application to be determined by the researcher.