DVL1 Antibody

ProSci
Product Code: PSI-13-706
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-706-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of Mouse kidney, using DVL1 antibody (13-706) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
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Immunohistochemistry of paraffin-embedded rat testis using DVL1 antibody (13-706) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse testis using DVL1 antibody (13-706) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of NIH/3T3 cells using DVL1 antibody (13-706) at dilution of 1:100. Blue: DAPI for nuclear staining.

Western blot analysis of extracts of Mouse kidney, using DVL1 antibody (13-706) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 60s.
Immunohistochemistry of paraffin-embedded rat testis using DVL1 antibody (13-706) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse testis using DVL1 antibody (13-706) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of NIH/3T3 cells using DVL1 antibody (13-706) at dilution of 1:100. Blue: DAPI for nuclear staining.

Further Information

Additional Names:
DVL1, DVL, MGC54245, DVL1L1, DVL1P1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
DVL1, the human homolog of the Drosophila dishevelled gene (dsh) encodes a cytoplasmic phosphoprotein that regulates cell proliferation, acting as a transducer molecule for developmental processes, including segmentation and neuroblast specification. DVL1 is a candidate gene for neuroblastomatous transformation. The Schwartz-Jampel syndrome and Charcot-Marie-Tooth disease type 2A have been mapped to the same region as DVL1. The phenotypes of these diseases may be consistent with defects which might be expected from aberrant expression of a DVL gene during development.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 510-640 of human DVL1 (NP_004412.2).
NCBI Gene ID #:
1855
NCBI Official Name:
dishevelled segment polarity protein 1
NCBI Official Symbol:
DVL1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 72kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction, Stem Cell
Swissprot #:
O14640
User NOte:
Optimal dilutions for each application to be determined by the researcher.