TST Antibody

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ProSci
Product Code: PSI-13-709
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-709-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TST antibody (13-709) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
2 / 2
Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug TST antibody (13-709). Western blot was performed from the immunoprecipitate using TST antibody (13-709) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using TST antibody (13-709) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug TST antibody (13-709). Western blot was performed from the immunoprecipitate using TST antibody (13-709) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
TST, MGC19578, RDS
Application Note:
WB: 1:1000 - 1:2000

IP: 1:50 - 1:100
Background:
This is one of two neighboring genes encoding similar proteins that each contain two rhodanese domains. The encoded protein is localized to the mitochondria and catalyzes the conversion of thiosulfate and cyanide to thiocyanate and sulfite. In addition, the protein interacts with 5S ribosomal RNA and facilitates its import into the mitochondria. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-297 of human TST (NP_003303.2).
NCBI Gene ID #:
7263
NCBI Official Name:
thiosulfate sulfurtransferase (rhodanese)
NCBI Official Symbol:
TST
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 36kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
Q16762
User NOte:
Optimal dilutions for each application to be determined by the researcher.