AP1S1 Antibody

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ProSci
Product Code: PSI-13-722
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-722-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of SW480 cells, using AP1S1 antibody (13-722) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
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Western blot analysis of extracts of SW480 cells, using AP1S1 antibody (13-722) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.

Documents

Further Information

Additional Names:
AP19, CLAPS1, EKV3, MEDNIK, SIGMA1A, AP-1 complex subunit sigma-1A, HA1 19 kDa subunit, adapter-related protein complex 1 sigma-1A subunit, adaptor protein complex AP-1 subunit sigma-1A, adaptor related protein complex 1 sigma 1 subunit, adaptor-related protein complex 1 subunit sigma-1A, clathrin assembly protein complex 1 sigma-1A small chain, clathrin coat assembly protein AP19, clathrin-associated/assembly/adaptor protein, small 1 (19kD), golgi adaptor HA1/AP1 adaptin sigma-1A subunit, sigma1A subunit of AP-1 clathrin adaptor complex, sigma1A-adaptin
Application Note:
WB: 1:500 - 1:1000
Background:
The protein encoded by this gene is part of the clathrin coat assembly complex which links clathrin to receptors in coated vesicles. These vesicles are involved in endocytosis and Golgi processing. This protein, as well as beta-prime-adaptin, gamma-adaptin, and the medium (mu) chain AP47, form the AP-1 assembly protein complex located at the Golgi vesicle.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-120 of human AP1S1 (NP_001274.1).
NCBI Gene ID #:
1174
NCBI Official Name:
adaptor related protein complex 1 subunit sigma 1
NCBI Official Symbol:
AP1S1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 19kDa
Purification:
Affinity purification
Research Area:
Immunology, Signal Transduction
Swissprot #:
P61966
User NOte:
Optimal dilutions for each application to be determined by the researcher.