MCM3 Antibody

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ProSci
Product Code: PSI-13-753
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-753-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MCM3 antibody (13-753) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded human oophoroma using MCM3 antibody (13-753) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using MCM3 antibody (13-753). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using MCM3 antibody (13-753) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human oophoroma using MCM3 antibody (13-753) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using MCM3 antibody (13-753). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
MCM3, HCC5, MGC1157, P1-MCM3, P1.h, RLFB
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is one of the highly conserved mini-chromosome maintenance proteins (MCM) that are involved in the initiation of eukaryotic genome replication. The hexameric protein complex formed by MCM proteins is a key component of the pre-replication complex (pre_RC) and may be involved in the formation of replication forks and in the recruitment of other DNA replication related proteins. This protein is a subunit of the protein complex that consists of MCM2-7. It has been shown to interact directly with MCM5/CDC46. This protein also interacts with and is acetylated by MCM3AP, a chromatin-associated acetyltransferase. The acetylation of this protein inhibits the initiation of DNA replication and cell cycle progression. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-295 of human MCM3 (NP_002379.2).
NCBI Gene ID #:
4172
NCBI Official Name:
minichromosome maintenance complex component 3
NCBI Official Symbol:
MCM3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 105kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
P25205
User NOte:
Optimal dilutions for each application to be determined by the researcher.