CSRP1 Antibody

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ProSci
Product Code: PSI-13-771
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-771-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CSRP1 Antibody (13-771) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
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Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug CSRP1 antibody (13-771). Western blot was performed from the immunoprecipitate using CSRP1 antibody (13-771) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using CSRP1 Antibody (13-771) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug CSRP1 antibody (13-771). Western blot was performed from the immunoprecipitate using CSRP1 antibody (13-771) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
CRP, CRP1, CSRP, CYRP, D1S181E, HEL-141, HEL-S-286, cysteine and glycine-rich protein 1, LIM-domain protein, cysteine-rich protein 1, epididymis luminal protein 141, epididymis secretory protein Li 286, epididymis secretory sperm binding protein
Application Note:
WB: 1:500 - 1:2000

IP: 1:50 - 1:200

RIP: 1:20 - 1:50
Background:
This gene encodes a member of the cysteine-rich protein (CSRP) family. This gene family includes a group of LIM domain proteins, which may be involved in regulatory processes important for development and cellular differentiation. The LIM/double zinc-finger motif found in this gene product occurs in proteins with critical functions in gene regulation, cell growth, and somatic differentiation. Alternatively spliced transcript variants have been described.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-193 of human CSRP1 (NP_004069.1).
NCBI Gene ID #:
1465
NCBI Official Name:
cysteine and glycine rich protein 1
NCBI Official Symbol:
CSRP1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 21kDa
Purification:
Affinity purification
Research Area:
Stem Cell, Transcription
Swissprot #:
P21291
User NOte:
Optimal dilutions for each application to be determined by the researcher.