POLR3A Antibody

ProSci
Product Code: PSI-13-776
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-776-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of 293T cells, using POLR3A antibody (13-776) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.

Western blot analysis of extracts of 293T cells, using POLR3A antibody (13-776) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.

Further Information

Additional Names:
ADDH, HLD7, RPC1, RPC155, WDRTS, hRPC155, DNA-directed RNA polymerase III subunit RPC1, DNA-directed RNA polymerase III largest subunit, DNA-directed RNA polymerase III subunit A, RNA polymerase III 155 kDa subunit, RNA polymerase III subunit C1, RNA polymerase III subunit C16RNA polymerase III subunit RPC155-D, polymerase (RNA) III (DNA directed) polypeptide A, 155kDa, polymerase (RNA) III subunit A
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200
Background:
The protein encoded by this gene is the catalytic component of RNA polymerase III, which synthesizes small RNAs. The encoded protein also acts as a sensor to detect foreign DNA and trigger an innate immune response.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-200 of human POLR3A (NP_008986.2).
NCBI Gene ID #:
11128
NCBI Official Name:
RNA polymerase III subunit A
NCBI Official Symbol:
POLR3A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 156kDa
Purification:
Affinity purification
Research Area:
Other
Swissprot #:
O14802
User NOte:
Optimal dilutions for each application to be determined by the researcher.