CUL3 Antibody

ProSci
Product Code: PSI-13-797
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-797-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CUL3 antibody (13-797) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human prostate using CUL3 antibody (13-797) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human gastric cancer using CUL3 antibody (13-797) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using CUL3 antibody (13-797). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using CUL3 antibody (13-797) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human prostate using CUL3 antibody (13-797) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human gastric cancer using CUL3 antibody (13-797) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CUL3 antibody (13-797). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
CUL3, cullin 3, KIAA0617
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:100

IF: 1:50 - 1:200
Background:
This gene encodes a member of the cullin protein family. The encoded protein plays a critical role in the polyubiquitination and subsequent degradation of specific protein substrates as the core component and scaffold protein of an E3 ubiquitin ligase complex. Complexes including the encoded protein may also play a role in late endosome maturation. Mutations in this gene are a cause of type 2E pseudohypoaldosteronism. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 569-768 of human CUL3 (NP_003581.1).
NCBI Gene ID #:
8452
NCBI Official Name:
cullin 3
NCBI Official Symbol:
CUL3
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 89-95kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle
Swissprot #:
Q13618
User NOte:
Optimal dilutions for each application to be determined by the researcher.