CREB1 Antibody [E0D3]

ProSci
Product Code: PSI-13-821
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-821-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Monoclonal
Antibody Clone: [E0D3]
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 6
Western blot analysis of extracts of various cell lines, using CREB1 antibody (13-821).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 6
Immunohistochemistry of paraffin-embedded mouse brain using CREB1 antibody (13-821) at dilution of 1:100 (40x lens).
3 / 6
Immunohistochemistry of paraffin-embedded human mammary cancer using CREB1 antibody (13-821) at dilution of 1:100 (40x lens).
4 / 6
Immunohistochemistry of paraffin-embedded human tonsil using CREB1 antibody (13-821) at dilution of 1:100 (40x lens).
5 / 6
Immunohistochemistry of paraffin-embedded human lung using CREB1 antibody (13-821) at dilution of 1:100 (40x lens).
6 / 6
Immunofluorescence analysis of HeLa cells using CREB1 antibody (13-821).

Western blot analysis of extracts of various cell lines, using CREB1 antibody (13-821).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded mouse brain using CREB1 antibody (13-821) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using CREB1 antibody (13-821) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using CREB1 antibody (13-821) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human lung using CREB1 antibody (13-821) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using CREB1 antibody (13-821).

Further Information

Additional Names:
CREB1, CREB, MGC9284
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:20 - 1:50
Background:
This gene encodes a transcription factor that is a member of the leucine zipper family of DNA binding proteins. This protein binds as a homodimer to the cAMP-responsive element, an octameric palindrome. The protein is phosphorylated by several protein kinases, and induces transcription of genes in response to hormonal stimulation of the cAMP pathway. Alternate splicing of this gene results in several transcript variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant protein of human CREB1
NCBI Gene ID #:
1385
NCBI Official Name:
cAMP responsive element binding protein 1
NCBI Official Symbol:
CREB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 37kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Immunology, Innate Immunity, Neuroscience, Signal Transduction, Transcription
Swissprot #:
P16220
User NOte:
Optimal dilutions for each application to be determined by the researcher.