MAGED1 Antibody

ProSci
Product Code: PSI-13-858
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-858-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MAGED1 antibody (13-858) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human liver cancer using MAGED1 antibody (13-858) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using MAGED1 antibody (13-858) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human liver cancer using MAGED1 antibody (13-858) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Melanoma-associated antigen D1, MAGE tumor antigen CCF, MAGE-D1 antigen, Neurotrophin receptor-interacting MAGE homolog, MAGED1, NRAGE
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
This gene is a member of the melanoma antigen gene (MAGE) family. Most of the genes of this family encode tumor specific antigens that are not expressed in normal adult tissues except testis. Although the protein encoded by this gene shares strong homology with members of the MAGE family, it is expressed in almost all normal adult tissues. This gene has been demonstrated to be involved in the p75 neurotrophin receptor mediated programmed cell death pathway. Three transcript variants encoding two different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 635-834 of human MAGED1 (NP_001005333.1).
NCBI Gene ID #:
9500
NCBI Official Name:
Melanoma-associated antigen D1
NCBI Official Symbol:
MAGED1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 86kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle
Swissprot #:
Q9Y5V3
User NOte:
Optimal dilutions for each application to be determined by the researcher.