BCL2 Antibody

ProSci
Product Code: PSI-13-890
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-890-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using BCL2 antibody (13-890) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
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Immunohistochemistry of paraffin-embedded human stomach using BCL2 antibody (13-890) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat pancreas using BCL2 antibody (13-890) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded rat brain using BCL2 antibody (13-890) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse pancreas using BCL2 antibody (13-890) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using BCL2 antibody (13-890) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded human stomach using BCL2 antibody (13-890) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat pancreas using BCL2 antibody (13-890) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using BCL2 antibody (13-890) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse pancreas using BCL2 antibody (13-890) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Bcl-2 Antibody: Bcl-2, PPP1R50, Apoptosis regulator Bcl-2
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IP: 1:50 - 1:100
Background:
This gene encodes an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human BCL2 (NP_000624.2).
NCBI Gene ID #:
596
NCBI Official Name:
B-cell CLL/lymphoma 2
NCBI Official Symbol:
BCL2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 28kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Autophagy, Cancer, Cell Cycle, Immunology, Neuroscience, Signal Transduction, Stem Cell
Swissprot #:
P10415
User NOte:
Optimal dilutions for each application to be determined by the researcher.