CTGF Antibody

ProSci
Product Code: PSI-13-902
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-902-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CTGF antibody (13-902) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded rat heart using CTGF antibody (13-902) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast using CTGF antibody (13-902) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse kidney using CTGF antibody (13-902) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of C6 cells using CTGF antibody (13-902) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of NIH/3T3 cells using CTGF antibody (13-902) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U2OS cells using CTGF antibody (13-902) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of U2OS cells using CTGF antibody (13-902) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of HeLa cells using CTGF antibody (13-902) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Western blot analysis of extracts of various cell lines, using CTGF antibody (13-902) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded rat heart using CTGF antibody (13-902) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast using CTGF antibody (13-902) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse kidney using CTGF antibody (13-902) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of C6 cells using CTGF antibody (13-902) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using CTGF antibody (13-902) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using CTGF antibody (13-902) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of U2OS cells using CTGF antibody (13-902) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of HeLa cells using CTGF antibody (13-902) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Further Information

Additional Names:
CCN2, NOV2, HCS24, IGFBP8, CCN2, Connective tissue growth factor, CCN family member 2, IBP-8
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:20 - 1:50
Background:
The protein encoded by this gene is a mitogen that is secreted by vascular endothelial cells. The encoded protein plays a role in chondrocyte proliferation and differentiation, cell adhesion in many cell types, and is related to platelet-derived growth factor. Certain polymorphisms in this gene have been linked with a higher incidence of systemic sclerosis.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 27-349 of human CTGF (NP_001892.1).
NCBI Gene ID #:
1490
NCBI Official Name:
connective tissue growth factor
NCBI Official Symbol:
CTGF
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 38kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Growth Factors, Immunology
Swissprot #:
P29279
User NOte:
Optimal dilutions for each application to be determined by the researcher.