IL18 Antibody, KO Validated

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ProSci
Product Code: PSI-13-933
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-933-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using IL18 antibody (13-933) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
2 / 3
Western blot analysis of extracts from normal (control) and IL18 knockout (KO) HeLa cells, using IL18 antibody (13-933) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug IL18 antibody (13-933). Western blot was performed from the immunoprecipitate using IL18 antibody (13-933) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using IL18 antibody (13-933) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
Western blot analysis of extracts from normal (control) and IL18 knockout (KO) HeLa cells, using IL18 antibody (13-933) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunoprecipitation analysis of 200ug extracts of HeLa cells, using 3 ug IL18 antibody (13-933). Western blot was performed from the immunoprecipitate using IL18 antibody (13-933) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
IL18, interleukin 18 (interferon-gamma-inducing factor), IGIF, IL-18, IL-1g, IL1F4, MGC12320, IL-1 gamma, interferon-gamma-inducing factor, interleukin 18, interleukin-1 gamma, interleukin-18
Application Note:
WB: 1:500 - 1:2000

IP: 1:50 - 1:100
Background:
The protein encoded by this gene is a proinflammatory cytokine that augments natural killer cell activity in spleen cells, and stimulates interferon gamma production in T-helper type I cells. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 37-193 of human IL18 (NP_001553.1).
NCBI Gene ID #:
3606
NCBI Official Name:
interleukin 18 (interferon-gamma-inducing factor)
NCBI Official Symbol:
IL18
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 23kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Chemokines & Cytokines, Growth Factors, Immunology, Innate Immunity
Swissprot #:
Q14116
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.