ANXA1 Antibody, KO Validated

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ProSci
Product Code: PSI-13-945
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-13-945-50uL50uL£505.00
Quantity:
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ANXA1 antibody (13-945).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 5
Western blot analysis of extracts from normal (control) and ANXA1 knockout (KO) HeLa cells, using ANXA1 antibody (13-945) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
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Immunohistochemistry of paraffin-embedded human esophagus using ANXA1 antibody (13-945) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse spleen using ANXA1 antibody (13-945) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using ANXA1 antibody (13-945). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using ANXA1 antibody (13-945).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts from normal (control) and ANXA1 knockout (KO) HeLa cells, using ANXA1 antibody (13-945) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human esophagus using ANXA1 antibody (13-945) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using ANXA1 antibody (13-945) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using ANXA1 antibody (13-945). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
ANXA1, ANX1, LPC1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene encodes a membrane-localized protein that binds phospholipids. This protein inhibits phospholipase A2 and has anti-inflammatory activity. Loss of function or expression of this gene has been detected in multiple tumors.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-346 of human ANXA1 (NP_000691.1).
NCBI Gene ID #:
301
NCBI Official Name:
annexin A1
NCBI Official Symbol:
ANXA1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 38kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
P04083
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.