MSH2 Antibody

Contact us
ProSci
Product Code: PSI-13-955
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-955-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of various cell lines, using MSH2 antibody (13-955) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
2 / 2
Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug MSH2 antibody (13-955). Western blot was performed from the immunoprecipitate using MSH2 antibody (13-955) at a dilition of 1:1000.
❮❯

Western blot analysis of extracts of various cell lines, using MSH2 antibody (13-955) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Immunoprecipitation analysis of 150ug extracts of A549 cells using 3ug MSH2 antibody (13-955). Western blot was performed from the immunoprecipitate using MSH2 antibody (13-955) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
MSH2, FCC1, COCA1, HNPCC, LCFS2, HNPCC1
Application Note:
WB: 1:500 - 1:2000

IP: 1:20 - 1:50
Background:
This locus is frequently mutated in hereditary nonpolyposis colon cancer (HNPCC). When cloned, it was discovered to be a human homolog of the E. coli mismatch repair gene mutS, consistent with the characteristic alterations in microsatellite sequences (RER+ phenotype) found in HNPCC. Two transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-300 of human MSH2 (NP_000242.1).
NCBI Gene ID #:
4436
NCBI Official Name:
mutS homolog 2
NCBI Official Symbol:
MSH2
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 105kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle
Swissprot #:
P43246
User NOte:
Optimal dilutions for each application to be determined by the researcher.