HDAC6 Antibody

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ProSci
Product Code: PSI-13-963
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-13-963-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using HDAC6 antibody (13-963) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunofluorescence analysis of HeLa cells using HDAC6 antibody (13-963) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
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Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug HDAC6 antibody (13-963). Western blot was performed from the immunoprecipitate using HDAC6 antibody (13-963) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using HDAC6 antibody (13-963) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunofluorescence analysis of HeLa cells using HDAC6 antibody (13-963) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunoprecipitation analysis of 100ug extracts of HepG2 cells using 3ug HDAC6 antibody (13-963). Western blot was performed from the immunoprecipitate using HDAC6 antibody (13-963) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
HDAC6 Antibody: HD6, JM21, CPBHM, KIAA0901, Histone deacetylase 6, HD6
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

IP: 1:20 - 1:50
Background:
Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 836-1104 of human HDAC6 (NP_006035.2).
NCBI Gene ID #:
10013
NCBI Official Name:
histone deacetylase 6
NCBI Official Symbol:
HDAC6
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 170kDa
Purification:
Affinity purification
Research Area:
Autophagy, Cell Cycle, Immunology, Signal Transduction, Stem Cell
Swissprot #:
Q9UBN7
User NOte:
Optimal dilutions for each application to be determined by the researcher.