LC3B Antibody, KO Validated

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ProSci
Product Code: PSI-13-971
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-13-971-50uL50uL£505.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 4
Western blot analysis of extracts of various cell lines, using LC3B antibody (13-971) at 1:1000 dilution. Both C6 cells and NIH/3T3 cells were treated by Chloroquine (50 uM) at 37°C for 20 hours.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3min.
2 / 4
Western blot analysis of extracts from normal (control) and LC3B knockout (KO) 293T cells, using LC3B antibody (13-971) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
3 / 4
Immunofluorescence analysis of HeLa cells using LC3B antibody (13-971) at dilution of 1:100. Hela cells were treated by Chloroquine (50 uM) for 20 hours(left). Blue: DAPI for nuclear staining.
4 / 4
Immunofluorescence analysis of NIH/3T3 cells using LC3B antibody (13-971) at dilution of 1:100. NIH/3T3 cells were treated by Chloroquine (50 uM) for 20 hours(left). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using LC3B antibody (13-971) at 1:1000 dilution. Both C6 cells and NIH/3T3 cells were treated by Chloroquine (50 uM) at 37°C for 20 hours.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 3min.
Western blot analysis of extracts from normal (control) and LC3B knockout (KO) 293T cells, using LC3B antibody (13-971) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of HeLa cells using LC3B antibody (13-971) at dilution of 1:100. Hela cells were treated by Chloroquine (50 uM) for 20 hours(left). Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using LC3B antibody (13-971) at dilution of 1:100. NIH/3T3 cells were treated by Chloroquine (50 uM) for 20 hours(left). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
MAP1LC3B Antibody: LC3B, ATG8F, MAP1LC3B-a, MAP1A/1BLC3, MAP1ALC3, Autophagy-related protein LC3 B
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The product of this gene is a subunit of neuronal microtubule-associated MAP1A and MAP1B proteins, which are involved in microtubule assembly and important for neurogenesis. Studies on the rat homolog implicate a role for this gene in autophagy, a process that involves the bulk degradation of cytoplasmic component.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide of human LC3B
NCBI Gene ID #:
81631
NCBI Official Name:
microtubule-associated protein 1 light chain 3 beta
NCBI Official Symbol:
MAP1LC3A/MAP1LC3B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 16kDa
Purification:
Affinity purification
Research Area:
Autophagy, Cancer, Cell Cycle, Neuroscience, Signal Transduction
Swissprot #:
Q9GZQ8
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.