IL1B Antibody

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ProSci
Product Code: PSI-14-002
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-002-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using IL1B antibody (14-002) at 1:1000 dilution. THP-1 cells were treated by PMA (80 nM) for overnight.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
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Immunofluorescence analysis of HeLa cells using IL1B antibody (14-002) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Immunofluorescence analysis of THP-1 cells using IL1B antibody (14-002) at dilution of 1:100. Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using IL1B antibody (14-002) at 1:1000 dilution. THP-1 cells were treated by PMA (80 nM) for overnight.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunofluorescence analysis of HeLa cells using IL1B antibody (14-002) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of THP-1 cells using IL1B antibody (14-002) at dilution of 1:100. Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
IL1B, interleukin 1, beta, IL-1, IL1-BETA, IL1F2, IL-1 beta, catabolin, interleukin-1 beta, preinterleukin 1 beta, pro-interleukin-1-beta
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200
Background:
The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 124-223 of human IL1B (NP_000567.1).
NCBI Gene ID #:
3553
NCBI Official Name:
interleukin 1, beta
NCBI Official Symbol:
IL1B
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 37kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Chemokines & Cytokines, Growth Factors, Immunology, Innate Immunity, Neuroscience, Obesity
Swissprot #:
P01584
User NOte:
Optimal dilutions for each application to be determined by the researcher.