c-Jun Antibody

Product Code: PSI-14-004

Supplier: ProSci

Code	Size	Price

PSI-14-004-50uL

50uL

£433.00

Quantity:

Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit

Antibody Isotype: IgG

Antibody Clonality: Polyclonal

Regulatory Status: RUO

Shipping:

Blue Ice or RT

Storage:

Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using c-Jun antibody (14-004) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.

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Immunohistochemistry of paraffin-embedded human colon using c-Jun antibody (14-004) at dilution of 1:100 (40x lens).

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Immunohistochemistry of paraffin-embedded human gastric cancer using c-Jun antibody (14-004) at dilution of 1:100 (40x lens).

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Immunohistochemistry of paraffin-embedded human lung using c-Jun antibody (14-004) at dilution of 1:100 (40x lens).

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Immunohistochemistry of paraffin-embedded human placenta using c-Jun antibody (14-004) at dilution of 1:100 (40x lens).

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Immunohistochemistry of paraffin-embedded human tonsil using c-Jun antibody (14-004) at dilution of 1:100 (40x lens).

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Immunohistochemistry of paraffin-embedded mouse testis using c-Jun antibody (14-004) at dilution of 1:100 (40x lens).

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Immunohistochemistry of paraffin-embedded rat brain using c-Jun antibody (14-004) at dilution of 1:100 (40x lens).

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Immunohistochemistry of paraffin-embedded rat liver using c-Jun antibody (14-004) at dilution of 1:100 (40x lens).

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Immunofluorescence analysis of U2OS cells using Histone H2AX antibody (13-997) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Immunofluorescence analysis of NIH/3T3 cells using c-Jun antibody (14-004) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Immunofluorescence analysis of U2OS cells using c-Jun antibody (14-004) at dilution of 1:100. Blue: DAPI for nuclear staining.

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Documents

Further Information

Additional Names:

JUN, AP1, AP-1, c-Jun

Application Note:

WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200

Background:

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Buffer:

PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

Concentration:

batch dependent

Conjugate:

Unconjugated

DISCLAIMER:

Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

Immunogen:

Recombinant fusion protein containing a sequence corresponding to amino acids 1-70 of human c-Jun (NP_002219.1).

NCBI Gene ID #:

3725

NCBI Official Name:

jun proto-oncogene

NCBI Official Symbol:

Jun

NCBI Organism:

Homo sapiens

Physical State:

Liquid

PREDICTED MOLECULAR WEIGHT:

Observed: 43kDa

Purification:

Affinity purification

Research Area:

Apoptosis, Cancer, Cell Cycle, Immunology, Innate Immunity, Signal Transduction, Transcription

Swissprot #:

P05412

User NOte:

Optimal dilutions for each application to be determined by the researcher.