FTH1 Antibody

ProSci
Product Code: PSI-14-022
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-022-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using FTH1 antibody (14-022) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 2
Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug FTH1 antibody (14-022). Western blot was performed from the immunoprecipitate using FTH1 antibody (14-022) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using FTH1 antibody (14-022) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation analysis of 200ug extracts of A-549 cells, using 3 ug FTH1 antibody (14-022). Western blot was performed from the immunoprecipitate using FTH1 antibody (14-022) at a dilition of 1:1000.

Further Information

Additional Names:
FTH1, FTH, FTHL6, MGC104426, PIG15, PLIF, FHC, HFE5
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:200

IP: 1:50 - 1:100
Background:
This gene encodes the heavy subunit of ferritin, the major intracellular iron storage protein in prokaryotes and eukaryotes. It is composed of 24 subunits of the heavy and light ferritin chains. Variation in ferritin subunit composition may affect the rates of iron uptake and release in different tissues. A major function of ferritin is the storage of iron in a soluble and nontoxic state. Defects in ferritin proteins are associated with several neurodegenerative diseases. This gene has multiple pseudogenes. Several alternatively spliced transcript variants have been observed, but their biological validity has not been determined.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-183 of human FTH1 (NP_002023.2).
NCBI Gene ID #:
2495
NCBI Official Name:
ferritin, heavy polypeptide 1
NCBI Official Symbol:
FTH1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 20kDa
Purification:
Affinity purification
Research Area:
Cancer, Neuroscience, Signal Transduction
Swissprot #:
P02794
User NOte:
Optimal dilutions for each application to be determined by the researcher.