NCF1 Antibody

ProSci
Product Code: PSI-14-032
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-032-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

1 / 2
Western blot analysis of extracts of various cell lines, using NCF1 antibody (14-032) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
2 / 2
Immunoprecipitation analysis of 200ug extracts of Raji cells, using 3 ug NCF1 antibody (14-032). Western blot was performed from the immunoprecipitate using NCF1 antibody (14-032) at a dilition of 1:1000.

Western blot analysis of extracts of various cell lines, using NCF1 antibody (14-032) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunoprecipitation analysis of 200ug extracts of Raji cells, using 3 ug NCF1 antibody (14-032). Western blot was performed from the immunoprecipitate using NCF1 antibody (14-032) at a dilition of 1:1000.

Further Information

Additional Names:
Ncf1, neutrophil cytosolic factor 1, NOXO2, Ncf-1, p47, p47phox, 47 kDa neutrophil oxidase factor, NADPH oxidase subunit (47 kD), NADPH oxidase subunit (47kDa), NCF-47K, OTTMUSP00000022112, neutrophil NADPH oxidase factor 1, neutrophil cytosol facto
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100

IP: 1:50 - 1:100
Background:
The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-390 of human NCF1 (NP_000256.4).
NCBI Gene ID #:
653361
NCBI Official Name:
neutrophil cytosolic factor 1
NCBI Official Symbol:
NCF1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 45kDa
Purification:
Affinity purification
Research Area:
Cancer, Signal Transduction
Swissprot #:
P14598
User NOte:
Optimal dilutions for each application to be determined by the researcher.