beta Catenin Antibody, KO Validated

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ProSci
Product Code: PSI-14-039
Product Group: Primary Antibodies
Supplier: ProSci
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PSI-14-039-50uL50uL£505.00
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Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using beta Catenin antibody (14-039) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
2 / 5
Western blot analysis of extracts from normal (control) and beta Catenin knockout (KO) HeLa cells, using beta Catenin antibody (14-039) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
3 / 5
Immunohistochemistry of paraffin-embedded human tonsil using beta Catenin antibody (14-039) at dilution of 1:100 (40x lens).
4 / 5
Immunohistochemistry of paraffin-embedded human mammary cancer using beta Catenin antibody (14-039) at dilution of 1:100 (40x lens).
5 / 5
Immunoprecipitation analysis of 200ug extracts of U-87MG cells, using 3 ug beta Catenin antibody (14-039). Western blot was performed from the immunoprecipitate using beta Catenin antibody (14-039) at a dilition of 1:1000.
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Western blot analysis of extracts of various cell lines, using beta Catenin antibody (14-039) at 1:3000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Western blot analysis of extracts from normal (control) and beta Catenin knockout (KO) HeLa cells, using beta Catenin antibody (14-039) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human tonsil using beta Catenin antibody (14-039) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human mammary cancer using beta Catenin antibody (14-039) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of U-87MG cells, using 3 ug beta Catenin antibody (14-039). Western blot was performed from the immunoprecipitate using beta Catenin antibody (14-039) at a dilition of 1:1000.

Documents

Further Information

Additional Names:
CTNNB1, CTNNB, MRD19, armadillo
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IP: 1:50 - 1:200
Background:
The protein encoded by this gene is part of a complex of proteins that constitute adherens junctions (AJs). AJs are necessary for the creation and maintenance of epithelial cell layers by regulating cell growth and adhesion between cells. The encoded protein also anchors the actin cytoskeleton and may be responsible for transmitting the contact inhibition signal that causes cells to stop dividing once the epithelial sheet is complete. Finally, this protein binds to the product of the APC gene, which is mutated in adenomatous polyposis of the colon. Mutations in this gene are a cause of colorectal cancer (CRC), pilomatrixoma (PTR), medulloblastoma (MDB), and ovarian cancer. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 1-100 of human beta Catenin (NP_001895.1).
NCBI Gene ID #:
1499
NCBI Official Name:
catenin (cadherin-associated protein), beta 1, 88kDa
NCBI Official Symbol:
CTNNB1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 105kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cancer, Cell Cycle, Immunology, Neuroscience, Signal Transduction, Stem Cell, Transcription
Swissprot #:
P35222
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.