CD31 Antibody

ProSci
Product Code: PSI-14-042
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-042-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CD31 antibody (14-042) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
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Immunohistochemistry of paraffin-embedded human liver cancer using CD31 antibody (14-042) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human colon using CD31 antibody (14-042) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human breast cancer using CD31 antibody (14-042) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using CD31 antibody (14-042) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.
Immunohistochemistry of paraffin-embedded human liver cancer using CD31 antibody (14-042) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human colon using CD31 antibody (14-042) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human breast cancer using CD31 antibody (14-042) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
Platelet endothelial cell adhesion molecule, PECAM-1, CD31, Pecam1, Pecam, Pecam-1
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IP: 1:50 - 1:200
Background:
The protein encoded by this gene is found on the surface of platelets, monocytes, neutrophils, and some types of T-cells, and makes up a large portion of endothelial cell intercellular junctions. The encoded protein is a member of the immunoglobulin superfamily and is likely involved in leukocyte migration, angiogenesis, and integrin activation.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 600 to the C-terminus of mouse CD31 (NP_001292086.1).
NCBI Gene ID #:
18613
NCBI Official Name:
Platelet endothelial cell adhesion molecule
NCBI Official Symbol:
PECAM1
NCBI Organism:
Mus musculus
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 130kDa
Purification:
Affinity purification
Research Area:
Cancer, Cell Cycle, Immunology, Signal Transduction, Stem Cell
Swissprot #:
P16284
User NOte:
Optimal dilutions for each application to be determined by the researcher.