MAD1L1 Antibody

ProSci
Product Code: PSI-14-043
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-043-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using MAD1L1 antibody (14-043) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
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Immunohistochemistry of paraffin-embedded mouse liver using MAD1L1 antibody (14-043) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human liver cancer using MAD1L1 antibody (14-043) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of U2OS cells using MAD1L1 antibody (14-043).

Western blot analysis of extracts of various cell lines, using MAD1L1 antibody (14-043) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
Immunohistochemistry of paraffin-embedded mouse liver using MAD1L1 antibody (14-043) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human liver cancer using MAD1L1 antibody (14-043) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of U2OS cells using MAD1L1 antibody (14-043).

Further Information

Additional Names:
Mitotic spindle assembly checkpoint protein MAD1, Mitotic arrest deficient 1-like protein 1, MAD1-like protein 1, Mitotic checkpoint MAD1 protein homolog, HsMAD1, hMAD1, Tax-binding protein 181, MAD1L1, MAD1, TXBP181
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
MAD1L1 is a component of the mitotic spindle-assembly checkpoint that prevents the onset of anaphase until all chromosome are properly aligned at the metaphase plate. MAD1L1 functions as a homodimer and interacts with MAD2L1. MAD1L1 may play a role in cell cycle control and tumor suppression. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 360-700 of human MAD1L1 (NP_001013859.1).
NCBI Gene ID #:
8379
NCBI Official Name:
Mitotic spindle assembly checkpoint protein MAD1
NCBI Official Symbol:
MAD1L1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 95kDa
Purification:
Affinity purification
Research Area:
Cell Cycle
Swissprot #:
Q9Y6D9
User NOte:
Optimal dilutions for each application to be determined by the researcher.