KDM1 Antibody, KO Validated

ProSci
Product Code: PSI-14-050
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-050-50uL50uL£505.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using KDM1 antibody (14-050) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
2 / 4
Western blot analysis of extracts from normal (control) and KDM1 knockout (KO) HeLa cells, using KDM1 antibody (14-050) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
3 / 4
Immunofluorescence analysis of A549 cells using KDM1 antibody (14-050).
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Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug KDM1 antibody (14-050). Western blot was performed from the immunoprecipitate using KDM1 antibody (14-050) at a dilition of 1:500.

Western blot analysis of extracts of various cell lines, using KDM1 antibody (14-050) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.
Western blot analysis of extracts from normal (control) and KDM1 knockout (KO) HeLa cells, using KDM1 antibody (14-050) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunofluorescence analysis of A549 cells using KDM1 antibody (14-050).
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug KDM1 antibody (14-050). Western blot was performed from the immunoprecipitate using KDM1 antibody (14-050) at a dilition of 1:500.

Further Information

Additional Names:
LSD1 Antibody: AOF2, KDM1, LSD1, BHC110, AOF2, KIAA0601, Lysine-specific histone demethylase 1A, BRAF35-HDAC complex protein BHC110
Application Note:
WB: 1:500 - 1:2000

IF: 1:50 - 1:100

IP: 1:50 - 1:100
Background:
This gene encodes a nuclear protein containing a SWIRM domain, a FAD-binding motif, and an amine oxidase domain. This protein is a component of several histone deacetylase complexes, though it silences genes by functioning as a histone demethylase. Alternative splicing results in multiple transcript variants.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 130-380 of human KDM1 (NP_055828.2).
NCBI Gene ID #:
23028
NCBI Official Name:
lysine (K)-specific demethylase 1
NCBI Official Symbol:
KDM1A
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 96-117kDa
Purification:
Affinity purification
Research Area:
Transcription
Swissprot #:
O60341
User NOte:
Optimal dilutions for each application to be determined by the researcher.
VALIDATION:
Antibody is Knockout validated.