TPM1 Antibody

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ProSci
Product Code: PSI-14-051
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-051-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using TPM1 antibody (14-051) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded human lung using TPM1 antibody (14-051) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human appendix using TPM1 antibody (14-051) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human uterus using TPM1 antibody (14-051) at dilution of 1:100 (40x lens).
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Immunofluorescence analysis of HeLa cells using TPM1 antibody (14-051). Blue: DAPI for nuclear staining.
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Western blot analysis of extracts of various cell lines, using TPM1 antibody (14-051) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human lung using TPM1 antibody (14-051) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human appendix using TPM1 antibody (14-051) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human uterus using TPM1 antibody (14-051) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using TPM1 antibody (14-051). Blue: DAPI for nuclear staining.

Documents

Further Information

Additional Names:
TPM1, CMH3, TMSA, TPM1-alpha, CMD1Y, LVNC9, C15orf13, HTM-alpha
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200

IF: 1:50 - 1:200
Background:
This gene is a member of the tropomyosin family of highly conserved, widely distributed actin-binding proteins involved in the contractile system of striated and smooth muscles and the cytoskeleton of non-muscle cells. Tropomyosin is composed of two alpha-helical chains arranged as a coiled-coil. It is polymerized end to end along the two grooves of actin filaments and provides stability to the filaments. The encoded protein is one type of alpha helical chain that forms the predominant tropomyosin of striated muscle, where it also functions in association with the troponin complex to regulate the calcium-dependent interaction of actin and myosin during muscle contraction. In smooth muscle and non-muscle cells, alternatively spliced transcript variants encoding a range of isoforms have been described. Mutations in this gene are associated with type 3 familial hypertrophic cardiomyopathy.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-245 of human TPM1 (NP_001018008.1).
NCBI Gene ID #:
7168
NCBI Official Name:
tropomyosin 1 (alpha)
NCBI Official Symbol:
TPM1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 36kDa
Purification:
Affinity purification
Research Area:
Cell Cycle, Signal Transduction
Swissprot #:
P09493
User NOte:
Optimal dilutions for each application to be determined by the researcher.