CHD4 Antibody

ProSci
Product Code: PSI-14-052
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-052-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using CHD4 antibody (14-052) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.

Western blot analysis of extracts of various cell lines, using CHD4 antibody (14-052) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 30s.

Further Information

Additional Names:
CHD4, ATP-dependent helicase CHD4, CHD-4, Mi-2b, Mi2-BETA
Application Note:
WB: 1:500 - 1:2000

IHC: 1:100 - 1:200
Background:
The product of this gene belongs to the SNF2/RAD54 helicase family. It represents the main component of the nucleosome remodeling and deacetylase complex and plays an important role in epigenetic transcriptional repression. Patients with dermatomyositis develop antibodies against this protein. Somatic mutations in this gene are associated with serous endometrial tumors. Alternative splicing results in multiple transcript variants encoding different isoforms.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1520-1690 of human CHD4 (NP_001264.2).
NCBI Gene ID #:
1108
NCBI Official Name:
chromodomain helicase DNA binding protein 4
NCBI Official Symbol:
CHD4
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 280kDa
Purification:
Affinity purification
Research Area:
Immunology
Swissprot #:
Q14839
User NOte:
Optimal dilutions for each application to be determined by the researcher.