ACTN1 Antibody

ProSci
Product Code: PSI-14-062
Product Group: Primary Antibodies
Supplier: ProSci
CodeSizePrice
PSI-14-062-50uL50uL£433.00
Quantity:
Prices exclude any Taxes / VAT

Overview

Host Type: Rabbit
Antibody Isotype: IgG
Antibody Clonality: Polyclonal
Regulatory Status: RUO
Shipping:
Blue Ice or RT
Storage:
Store at -20°C. Avoid freeze / thaw cycles.

Images

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Western blot analysis of extracts of various cell lines, using ACTN1 antibody (14-062) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
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Immunohistochemistry of paraffin-embedded human liver cancer using ACTN1 antibody (14-062) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded human esophagus using ACTN1 antibody (14-062) at dilution of 1:100 (40x lens).
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Immunohistochemistry of paraffin-embedded mouse heart using ACTN1 antibody (14-062) at dilution of 1:100 (40x lens).

Western blot analysis of extracts of various cell lines, using ACTN1 antibody (14-062) at 1:500 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunohistochemistry of paraffin-embedded human liver cancer using ACTN1 antibody (14-062) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human esophagus using ACTN1 antibody (14-062) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse heart using ACTN1 antibody (14-062) at dilution of 1:100 (40x lens).

Further Information

Additional Names:
ACTN1, FLJ40884, BDPLT15
Application Note:
WB: 1:500 - 1:2000

IHC: 1:50 - 1:200
Background:
Alpha actinins belong to the spectrin gene superfamily which represents a diverse group of cytoskeletal proteins, including the alpha and beta spectrins and dystrophins. Alpha actinin is an actin-binding protein with multiple roles in different cell types. In nonmuscle cells, the cytoskeletal isoform is found along microfilament bundles and adherens-type junctions, where it is involved in binding actin to the membrane. In contrast, skeletal, cardiac, and smooth muscle isoforms are localized to the Z-disc and analogous dense bodies, where they help anchor the myofibrillar actin filaments. This gene encodes a nonmuscle, cytoskeletal, alpha actinin isoform and maps to the same site as the structurally similar erythroid beta spectrin gene. Three transcript variants encoding different isoforms have been found for this gene.
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Conjugate:
Unconjugated
DISCLAIMER:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-330 of human ACTN1 (NP_001123477.1).
NCBI Gene ID #:
87
NCBI Official Name:
actinin, alpha 1
NCBI Official Symbol:
ACTN1
NCBI Organism:
Homo sapiens
Physical State:
Liquid
PREDICTED MOLECULAR WEIGHT:
Observed: 105kDa
Purification:
Affinity purification
Research Area:
Apoptosis, Cell Cycle, Immunology, Signal Transduction, Stem Cell
Swissprot #:
P12814
User NOte:
Optimal dilutions for each application to be determined by the researcher.